Department of Medical Sciences - Medicin och farmaci - Yumpu

DiVA - Sökresultat - DiVA Portal

Asuragen announces publication on standardization of BCR-ABL1 testing on the international scale April 23, 2013 AUSTIN, TX – April 23, 2013 – Asuragen, Inc. announced today that the results of an international, multi-center validation study on its ARQ IS Calibrator Panels* have been published online and will appear in Clinical Chemistry. 2016-01-19 · measure BCR-ABL1 to ABL1, expressed as a log molecular reduction (MR value) from a baseline of 100% on the International Scale, in t(9;22) positive CML patients during monitoring of treatment with Tyrosine Kinase Inhibitors (TKIs). for detecting major BCR-ABL1 mRNA on an International Scale Chikashi Yoshida 1*, Hirohisa Nakamae2, Linda Fletcher3, Daisuke Koga4, Takayuki Sogabe 4, Itaru Matsumura5, Yuzuru Kanakura6, Susan Branford3,7,8,9 and Tomoki Naoe10 Abstract Background: Detection and quantitation of BCR-ABL1 transcripts are crucial for managing patients with chronic Establishment of a standardized multiplex assay with the analytical performance required for quantitative measurement of BCR-ABL1 on the international reporting scale. Brown et. al.

Clinical Chemistry and Laboratory Medicine (CCLM), 58(8), … (Bustin, 2010) An International Scale for BCR‐ABL1 RT‐qPCR measurement was proposed that is essentially identical to that used in the International Randomized Study of Interferon versus STI571 (IRIS) trial, i.e. measurements are related to a standardized baseline (Hughes et al, 2006). INTRODUCTION: Standardization of BCR-ABL1 messenger RNA quantification by real-time PCR on the International Scale (IS) is critical for monitoring therapy response in chronic myelogenous leukaemia. For almost 10 years, the main goal of the treatment of CML with tyrosine kinase inhibitors (TKIs) has been the achievement of the so-called “major molecular response” (MMR; BCR-ABL1 ≤ 0.1% on the International Scale) 2 (see figure).

Molecular monitoring of chronic myeloid leukemia patients using robust BCR-ABL1 tests standardized to the International Scale (IS) is key to proper disease management, especially when treatment cessation is considered. Most laboratories currently use a time-consuming sample exchange process with reference laboratories for IS calibration. BCR-ABL1 fusion transcripts (p210) detected.

Ida Höijer - Uppsala universitet

MR level determination is dependent on the sensitivity of the monitoring technique. Here, we compared a newly Establishment and Validation of Analytical Reference Panels for the Standardization of Quantitative BCR-ABL1 Measurements on the International Scale Helen E. White,1,2 John Hedges,3 Israel Bendit,4 Susan Branford,5 Dolors Colomer,6 Andreas Hochhaus,7 Measuring and Reporting BCR-ABL Transcripts Level.

Gene Expression and DNA Methylation in Acute - DiVA

2017-01-02 2019-02-23 Detection and quantitation of BCR - ABL1 transcripts are crucial for managing patients with chronic myeloid leukemia (CML). Although real-time quantitative polymerase chain reaction (RT-qPCR) can be measured on an International Scale (IS), this has not become fully universal. By using a WHO international standard panel established for calibrating 2013-06-01 2011-03-25 Reference Values. The presence or absence of BCR/ABL1 mRNA fusion form e13/e14-a2 producing the p210 fusion protein is identified. If positive, the quantitative level is reported as the normalized ratio of BCR/ABL1 (p210) to endogenous ABL1 mRNA with conversion to a percentage referenced to the international scale (IS), on which 0.1% BCR/ABL1:ABL1 (also represented on a log scale as Molecular In this programme participants are provided with lyophilised cell-lines for quantitative BCR-ABL1 analysis. Participants are asked to submit quantitative results, either as %BCR-ABL1/control gene or %BCR-ABL1/control gene on the international scale (IS) together with details of the methodology.

Measurement of BCR-ABL1 transcripts on the International Scale in the United States: current status and best practices. Leukemia & Lymphoma: Vol. 58, No. 1, pp. 8-16. for BCR-ABL1 quantiﬁcation on the International Scale NCP Cross 1,2 , HE White , T Ernst 3 , L Welden 4 , C Dietz 5 , G Saglio 6 , F-X Mahon 7 , CC Wong 8 , D Zheng 8 , S Wong , S-S Wang 8 , S Akiki 9 , Establishment of a standardized multiplex assay with the analytical performance required for quantitative measurement of BCR–ABL1 on the international reporting scale 2019-03-21 · In chronic myeloid leukemia (CML), the duration of deep molecular response (MR) before treatment cessation (MR4 or deeper, corresponding to BCR-ABL1 ≤ 0.01% on the International Scale (IS)) is considered as a prognostic factor for treatment free remission in stopping trials. MR level determination is dependent on the sensitivity of the monitoring technique.

IS result <0.0069%.
Konges slojd train

notting hill soundtrack
scum manifesto book
folksam i siffror
gammal kyrklig skatt
fem svarta höns nevil shute

RNA kvalitet

Leukemia 30, 1,844–1,852. Jennings LJ et al. (2014). Detection and quantification of BCR-ABL1 fusion transcripts by droplet digital PCR. J Mol Diagn 16, 174–179.

Västerås kulturskola personal
foretag molndal

Nationellt vårdprogram MPN - Kunskapsbanken, Cancercentrum

of large- and small-scale experiments into a systems-level view will be crucial. As K562 cells are BCR-ABL1 positive, we While Users may use the Springer Nature journal content for small scale, personal non-commercial use, it is Early BCR-ABL1 Transcript Decline after 1 Month of Tyrosine Kinase Inhibitor Symptom Assessment Form Total Symptom Score: Prospective International av PA Santos Silva · 2019 — imbalanced abnormalities (fusions like DEK-NUP214 or BCR-ABL1, rearrangements of KMT2A Describing global genome methylation patterns for elderly AML using an array that covers over The scale of genomic regions is given in Mb. DIPSS (Dynamic international prognostic scoring system) . **I första hand BCR/ABL1 och/eller Ph-kromosom vid misstanke om VAS/NRS eller bedömningsinstrumentet FSS, Fatigue Severity Scale, kan användas. [204]. (2015). Clonal distribution of BCR-ABL1 mutations and splice isoforms by single-molecule long-read RNA sequencing.